Coding

Part:BBa_K572009

Designed by: Kousik Sundararajan   Group: iGEM11_IIT_Madras   (2011-10-03)

Mouse Beta Carotene Dioxygenase

It is an oxidoreductase-enzyme which catalyzes the conversion of beta-carotene to retinal in the presence of oxygen, according to the reaction:
beta-carotene + O2 <=> 2 retinal.
The product of this enzymatic reaction, i.e Retinal is a VitaminA aldehyde. It is a polyene chromophore which binds to proteins called 'Opsins' (say Proteorhodopsin). This retinal-opsin complex allows microorganisms to convert light energy to metabolic energy. Hence in micro-organisms functionally expressing Proteorhodopsin, beta-carotene dioxygenase converts beta-carotene to Trans-retinal, which is required for the functioning of Proteorhodopsin.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 82
    Illegal PstI site found at 220
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 82
    Illegal PstI site found at 220
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1443
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 82
    Illegal PstI site found at 220
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 82
    Illegal PstI site found at 220
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 418
    Illegal SapI site found at 999


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